GSM5172708: SubAa272_rep2; Mus musculus; RNA-Seq - SRA

SRX10344545: GSM5172708: SubAa272_rep2; Mus musculus; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 21.3M spots, 6.4G bases, 1.8Gb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing Facilitates analysis of MODE-K SubAa272 and control cells genes expression

PRJNA714644 • SRP310712 • All experiments • All runs

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We performed the bulk RNA seq on the MODE-K SubAa272 and Control stable cell line and found the different genes expression in the setting of SubAa272 caused ER stress. Overall design: The RNA were extracted from 1x10^6 MODE-K SubAa272 and Control stable cells by RNeasy Plus Mini Kit(Qiagen,74134). Then Library preparation and transcriptome sequencing on an Illumina HiSeq X Ten platform were performed by Novogene Bioinformatics Technology Co., Ltd. to generate 150-bp paired-end reads.

Sample: SubAa272_rep2

SAMN18314017 • SRS8463663 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 1x10^6 MODE-K SubAa272 and Control stable cells were harvasted and extracted by RNeasy Plus Mini Kit(Qiagen,74134). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5172708

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 21.3M spots, 6.4G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR13966622	21,326,869	6.4G	1.8Gb	2023-03-16

ID:: 13537497

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